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Forum 15.1［PDF 3.18MB］

EPICENTRE Forum : アーカイブ

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2008

Forum 15 . 1［PDF 3.18MB］

• Rapid, Easy Extraction of DNA and RNA from Archival Samples
• Direct Isolation of Metagenomic DNA from Environmental Water Samples
• A Sense-RNA Amplification Method that Preserves Both 3'and 5' Transcript Information
• Fast PCR with Long and Difficult Amplicons
• Greatly Improved Real-Time RT-PCR from Small Quantities of Degraded RNA Samples
• Ask Frank - Production and Labeling of Antisense RNA using EPICENTRE’s TargetAmp™ Kits
• Product Highlights

2007

Forum 14 . 2［PDF 2.94MB］

• ExactStart™ Full-Length cDNA Library Cloning Kit: A Rapid and Efficient Method to Synthesize Full-Length cDNA for Cloning and Accurate Mapping of Transcription Initiation and Polyadenylation Sites
• Fast Real-Time PCR with a Standard qPCR Thermocycler!
• Easy and Rapid Extraction of Plant DNA Using the QuickExtract™ Plant DNA Extraction Solution
• Ask Frank - Questions about RNA capping and in vitro transcription
• A Simple Enzymatic Process for Generating mRNA-Enriched Poly(A)-Tailed RNA from Total RNA Samples
• Protein Expression from a Chromosomally-Inserted Transposon
• Isolate Intron Lariat Loops with RNase R
• Talk Tidbits

Forum 14 . 1［PDF 2.87MB］

• The New mScript™ mRNA Production System - Efficient mRNA Transcription, Capping and Tailing for the Highest Yields of Active Protein
• Detect More Transcripts and Use Less RNA in Your Microarray Studies; TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 104
• A Sensitive Method For 5´-Terminal End-group Analysis by Terminator™ 5´-Phosphate-Dependent Exonuclease
• Product Data Sheets for EPICENTRE Biotechnologies’ Products
• Ask Frank, by Fred Hyde and Hank Daum
• Synthesis of Full-Length cDNA Greater Than 15 kb for qPCR Using the New MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit
• Rapid Sampling and Identification of Mitochondrial and Genomic DNA
• PCR Analysis of Fingernail DNA Using QuickExtract™ DNA Extraction Solution
• Make Circular ssDNA in 1 Hour Without Using Oligo “Bridges”
• Biotin-16-UTP Now Available
• Prepare Labeled RNA Probes More Efficiently and Economically Using the AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit
• Also Available: Biotin-X-X-NHS

2006

Forum 13 . 4［PDF 1.6MB］

• Achieve 100% Capping Efficiency with the NEW ScriptCap™ m7G Capping System
• Improve the Translation Efficiency of any 5'-Capped mRNA with the NEW ScriptCap™ 2'-O-Methyltransferase
• High Sensitivity RT-PCR from as Little as 1 Cell
• Direct Genomic DNA Sequencing for Rapid Fungal Identification
• Ask Frank
• PCR Amplification of a High-GC Region of the Glucocorticoid Receptor Gene Using the FailSafe™ PCR System
• 3'/5' Ratio Analysis of FFPE RNA Purified by the MasterPure™ RNA Purification Kit
• Rapid and Highly Sensitive Screening of Bacterial RNA Polymerase Activity and Inhibitors Using the Kool™ NC-45™ Universal RNA Polymerase Templates
• Obtain Higher Molecular Weight Soil DNA without Bead-Beating

Forum 13 . 3［PDF 2.5MB］

• High-Throughput Isolation of BAC DNA That is Free of E. coli Genomic DNA and RNA
• The EZ-Tn5™ Transposome™ Complex Simplifies Microbial Engineering
• Achieve High Fidelity, High Sensitivity, and High Yield RNA Amplification That Produces High Quality Array Results
• In 30 Minutes Obtain PCR-Ready DNA from FFPE Tissue with QuickExtract™ DNA Extraction Solution
• Ask Frank
• FailSafe™ PROBES Real-Time PCR Optimization Kit Greatly Improves Multiplex Performance
• Extensive Range of Plant Genomic Libraries Made with the CopyControl™ Fosmid Library Production Kit
• Sensitive qRT-PCR Obtained from the Lysates of 1 - 10 Cells Using the MessageBOOSTER™ cDNA Synthesis Kit for qPCR

Forum 13 . 2［PDF 1.6MB］

• The New MessageBOOSTER™ cDNA Synthesis Kit for qPCR Preserves Transcript Abundance
• Produce High Yields of a High Affinity 2’-F-RNA Aptamer Using the DuraScribe® T7 Transcription Kit
• Control the Length of the 3’-Poly(A)-tail Added to Your RNA Using the A-Plus™ Poly(A) Polymerase Tailing Kit
• Ask Frank
• The High Yield ArrayPure™ Nano-scale RNA Purification Kit Outperforms Kits from Two Other Major Suppliers
• A Rapid Method for Extraction of Rotavirus RNA from Fecal Samples and Genotyping of Rotavirus by Reverse Transcription-PCR
• Get the Highest Yield of 5’-Capped RNA from an In Vitro Transcription Reaction
• Recent Publications Citing the TargetAmp™ aRNA Amplification Kits for Gene Expression Studies
• Tobacco Acid Pyrophosphatase Efficiently Removes the 5’-Cap Structure From Eukaryotic mRNAs
• Impressive Reproducibility of ArrayPure™ RNA Purifications

Forum 13 . 1［PDF 3.00MB］

• TargetAmp™ aRNA Amplification Kits: High Fidelity RNA Amplification for High Quality Microarray Results
• Rapid, High Yield Purification of Plasmids Free of RNA and Genomic DNA
• MessageBOOSTER™ cDNA Synthesis Kit for Real-Time PCR Quantification of Low-Abundance Transcripts from as Little as One Cell
• An Improved CopyControl™ Fosmid Vector Maximizes End-Sequencing Results
• The Generation of Alu-Based DNA Profiles Using EPICENTRE’s FailSafe™ PCR System
• Molecular Analysis of Lactic Acid Bacteria in an Inhospitable Environment: Beer
• Ask Frank
• Enrich Prokaryotic or Eukaryotic mRNA Using EPICENTRE’s Terminator™ 5’-Phosphate-Dependent Exonuclease
• Make Circular ssDNA without “Bridges” or “Splints”

2005

Forum 12 . 1［PDF 1.95MB］

• TargetAmp™ 2-Round Kits: An Improved RNA Linear Amplification System for Microarray Analysis from as Little as 1 Cell
• Eberwine-type Kits Generate High Yields of aRNA from 25 to 500 ng of Total RNA
• Biotin-X-X-NHS, for Biotin Labeling of Aminoallyl-RNA
• Purify RNA from a Single Cell Using the ArrayPure™ Nano-scale RNA Purification Kit
• Ask Frank
• FailSafe™ PROBES Real-Time PCR System Enables Easy, Reliable Optimization of Allelic Descrimination Assays
• TargetAmp™ Aminoallyl-aRNA is a Suitable Template for cDNA Synthesis
• Full-Length Reverse Transcription of >15-kb mRNA Using MonsterScript™ Reverse Transcriptase
• Fecal DNA is PCR-Ready in 50 Minutes Using the ExtractMaster™ Fecal DNA Extraction Kit

2004

Forum 11 . 6［PDF 1.96MB］

• Purify Microbial DNA from Water Samples Using the WaterMaster™ DNA Purification Kit
• Purify RNA from 10 to 10,000 Eukaryotic Cells Using the ArrayPure™ Nano-scale RNA Purification Kit
• DNA Isolation from a Filamentous Fungus Using the MasterPure™ Yeast DNA Purification Kit
• Environmental Genomics Studies Use EPICENTRE's Fosmid Kits to Generate Libraries
• Quickly Extract DNA from Tissue in 8 Minutes
• Ask Frank
• Transposon-Based Method to Create and Express N-Terminal and C-Terminal Protein Deletions
• Rapidly Screen Bacterial RNA Polymerase Inhibitors Using Innovative Kool™ NC-45™ Technology
• Transcribe ssDNA Templates with Single-Stranded Promoters Using the MiniV™ In Vitro Transcription Kit
• Produce Templates for Rolling Circle Replication and Transcription in 1 Hour Using CircLigase™ ssDNA Ligase

Forum 11 . 5［PDF 1.21MB］

• APex™ Heat-Labile Alkaline Phosphatase Dephosphorylates Any DNA Ends in Just 15 Minutes
• Clone Unstable DNA by Lowering the Copy Number of Common Vectors Using CopyCutter ™ EPI400™ E. coli Cells
• Undergraduates Study Gene Expression in S. cerevisiae Using a Student-Optimized Protocol
• Introducing TAQurate™ Real Time PCR Master Mix, A Single High-Performance Mix
• Ask Frank
• Restriction-Enzyme Screen Clones from Colony to Gel in 25 Minutes or Less
• Colony Fast-Screen™ Kit (Restriction Screen) Identifies Subclones with 124-bp Fragment
• The Oyster Bacterial Biome: Who Else Is Inside the Shell?
• Transposon Construction Vectors
• Tobacco Acid Pyrophosphatase (TAP) Functional Assay Ensures Decapped mRNA

Forum 11 . 4［PDF 3.43MB］

• 3-Minute Protocol for the BuccalAmp™ DNA Extraction Kit
• Customer Focus
• “Fishing” for Genes Encoding Noncytoplasmic Proteins in a Vibrio Fosmid Library
• Ask Frank
• Cloning a Real-Time PCR Product; Does SYBR® Green I Dye Interfere?
• High Throughput Screening For RNAi Using the MessageMuter™ shRNAi Production Kit
• Screen BAC Clones Quickly and Efficiently, Without Not I

Forum 11 . 3［PDF 3.55MB］

• Isolate BAC DNA Pure Enough to Store for One Year Using the BACMAX™ DNA Purification Kit
• Introducing MasterPure™ Gram Positive DNA Purification Kit
• A Rapid Method to Assess Protein Overproduction and Solubility in E. coli Using EasyLyse™ Bacterial Protein Extraction Solution
• Transcribe High Yields of Active Ribozyme in 30 Minutes Using the AmpliScribe™ T7 -Flash™ Transcription Kit
• Ask Frank
• Customer Focus
• Cloning and Expression of Moraxella catarrhalis Adhesins Using the CopyControl™ PCR Cloning System
• Transposon-Based Strategies for Efficient DNA Sequencing and Functional Genomics

Forum 11 . 2［PDF 1.89MB］

• A Novel, Rapid Method to Release Intact Yeast RNA Using the MasterPure™ Yeast RNA Purification Kit
• High Efficiency Real-Time PCR Amplifies a 1.6-kb Template Using the FailSafe™ Real-Time PCR System
• Ask Frank
• Customer Focus
• shRNA Offers Important Advantages Over siRNA for RNAi Studies: MessageMuter™ shRNAi Production Kit
• Generate High Yields of Biotin-Labeled RNA Using the AmpliScribe™ T7-Flash™ or the AmpliScribe™ T3-Flash™ Transcription Kits

Forum 11 . 1［PDF 2.99MB］

• RNAi Studies Made Faster, Easier, and at Lower Cost Using the New MessageMuter™ shRNAi Production Kit
• Improve Your RT-PCR Results Using Thermostable MasterAmp™ Tth DNA Polymerase
• Maximize the Yield of Short RNA Transcripts Using an AmpliScribe™ T7-Flash™ or T3-Flash™ Transcription Kit
• Ask Frank
• Customer Focus
• Prepare DNA from Colonies in 10 Minutes for Real-Time PCR Using the Colony Fast-Screen™ Kit and FailSafe™ Real-Time PCR System
• Get High Stringency and High Specificity Ligation-Dependent Mutation Screening with Ampligase® Thermostable DNA Ligase
• Transcribe 2’-Modified RNAs, Including RNAs Resistant to RNase A, Using Mutant T7 and SP6 R&DNA™ Polymerases
• Determining the Copy Number of Induced CopyControl™ Clones Using the FailSafe™ Real-Time PCR System

2003

Forum 10 . 3［PDF 4.88MB］

• The FailSafe™ Real-Time PCR Capillary System: Now Available for Use with Capillary-Based Real-Time PCR Instruments
• DNA Purification from Hard-to-Lyse Bacteria Using Ready-Lyse™ Lysozyme and the MasterPure™ DNA Purification Kit
• Introducing EasyLyse™ Bacterial Protein Extraction Solution to Gently Lyse Cells While Preserving up to 10 Times More Enzyme Activity of Purified Proteins
• Quantifying RNAi-Meditated Gene Silencing by Real-Time RT-PCR Using the MasterAmp™ Real-Time RT-PCR Kit
• Transcribe Milligram Amounts of RNA in as Little as 30 Minutes Using the AmpliScribe™ T7-Flash™ Transcription Kit
• Ask Frank
• Customer Focus
• The EZ::TN™ Transposome™ Complex Simplifies Transposon Mutagenesis in a Wide Variety of Microorganisms
• Brian Wickes, University of Texas Health Science Center at San Antonio, a MasterPure™ Yeast DNA Purification Customer, Outlines His Fungal Research
• EPICENTRE Completes Validation of New cGMP Compliant Manufacturing Facility

Forum 10 . 2［PDF 1.48MB］

• Rapid, High Yield Transcription of Short Hairpin RNA (shRNA) for RNAi-Mediated Gene Silencing
• High Quality RNA for Use with Microarrays
• MasterPure™ Genomic DNA for Bacillus anthracis Analysis
• DuraScript™ dsRNA Digested Using Human Dicer Enzyme Is As Effective and Specific in RNAi-Mediated Gene Silencing As Canonical "Diced" dsRNA
• Transcribe More RNA in 30 Minutes Than Other In Vitro Transcription Kits Produce in 2 Hours
• Obtain PCR-Ready Genomic DNA from Buccal Cells, HeLa Cells, Hair Follicles, Tail Snips, Bacterial Cells, or Feathers Using the QuickExtract™ DNA Extraction Solution
• 10 Reasons Why DNA Cloning Kits from EPICENTRE Enable the Fastest and Most Reliable Results
• Efficient Cloning of Entire Mitochondrial Genomes in Escherichia coli by In Vitro Insertion of a Transposon
• CopyControl™ Cloning Systems Allow Single-Copy Cloning, Then Controlled Induction for High-Copy DNA Production
• The FailSafe™ Real-Time PCR System with SYBR® Green I Dye Provides Shorter Cycle Times and More Consistent PCR Quantitation with Every Template Every Time

Forum 10 . 1［PDF 1.21MB］

• The FailSafe™ Real-Time PCR System Provides Highly Sensitive and Specific Real-Time PCR Analysis
• The MasterAmp™ Real-Time RT-PCR Kit Provides Superior Sensitivity and Consistent Quantitation
• 6 Reasons Why DuraScript™ RNA Is Better for RNAi
• Production of High-Activity Digoxigenin-Labeled Riboprobes for In Situ Hybridization Using the AmpliScribe™ T7 High Yield Transcription Kit
• Transcribe More RNA in 30 Minutes Than the Leading Competitor's "High Yield" Transcription Kit Can Do in 2 Hours
• Obtain the Highest Yield of Capped RNA from an In Vitro Transcription Reaction
• The SoilMaster™ DNA Extraction Kit Provides PCR-Ready Soil DNA in Less Than an Hour
• Which Kit is Best for Your Genomic DNA Cloning Project-the CopyControl™ Fosmid Library Production Kit or the CopyControl™ BAC Cloning Kit?
• Now, There Are Two Ways to Rapidly Screen Your Clones without Cultures, DNA Purifications, or Restriction Digests
• Direct Selection of Genes Encoding Non-Cytoplasmic Proteins
• Efficient Mapping of Functional Domains or Epitopes of Proteins
• A Mu-Based Transposition System that Is At Least 50-Fold More Efficient than the Competition
• Phage T1-Resistant Electrocompetent E.coli with a Transformation Efficiency of >1 X 1010

2002

Forum 9 . 3［PDF 912KB］

• Construction of Four CopyControl™ BAC Libraries by BACTROP - a BAC-Based Platform to Study Tropical Plant Species
• How the CopyControl™ BAC Cloning Kits Work
• Unlike Other PCR Products, the FailSafe™ PCR System Amplifies a GC-Rich Template and a 20-Kb Template on the First Try
• Jumping Green Genes: A Transposon-Based Approach for Rapidly Creating Functional, Flourescent Fusion Proteins
• Simple, Efficient Production of Short Double-Stranded RNA Using RNase III
• RNase III (E.coli) Now Available from EPICENTRE
• Get Consistently High Yields of Full-Length RNA from Both Long and Short DNA Templates Using AmpliScribe™ T7 High Yield Transcription Kits
• TransforMax™ EC100™ Chemically Competent E.coli Beat the Competition in Transformation Efficiency
• Quick and Efficient Purification of Single- or Double-Stranded RNA Transcripts Using the MasterPure™ RNA Purification Kit
• Obtain the Highest DNA Yields from Yeast with the MasterPure™ Yeast DNA Purification Kit
• Ampligase® Thermostable DNA Ligase
• Get Cleaner Plasmid Preparations with Plasmid-Safe™ ATP-Dependent DNase
• Increase Protein Yield for Proteomics, High-Throughput Screening or Preparative Purification
• Identification of a Potential Virulence-Related Operon by Rescue Cloning EZ::TN™ Transposon Insertion Sites from a Bacterial Pathogen

Forum 9 . 2［PDF 1.01MB］

• Extract PCR-Ready Soil DNA in Less Than an Hour with the New SoilMaster™ DNA Extraction Kit
• Here is How to Never Fail at PCR
• Rapid and Efficient Cloning of Large PCR Products, Up to 15 Kb, Using the New CopyControl™ PCR Cloning Kits
• Rapid Identification of EZ::TN™ Transposon Insertion Sites in the Genome of Neisseria gonorrhoeae
• TypeOne™ Inhibitor Improves Transformation Efficiencies by Blocking Type I Restriction and Modification Systems In Vivo
• n Improved Approach for Construction and Analysis of BAC Libraries Using the CopyControl™ pCC1BAC™ Vector Containing an Inducible High-Copy Origin
• Integrate CopyControl™ Capability into Existing BAC and Fosmid Clones
• Size Your BAC Clones in 4 Hours without Growing Cultures or Performing Restriction Digests
• Isolation of Bovine and Mouse DNA from Buccal and Vaginal Samples Using the BuccalAmp™ DNA Extraction Kit
• Science Students Amplify Human Globin Gene DNA from Buccal Samples
• Get Consistently High Yields of Full-Length RNA From Both Long and Short DNA Templates Using AmpliScribe™ T7 High Yield Transcription Kits

Forum 9 . 1［PDF 896KB］

• EPICENTRE Revolutionizes Cloning By Introducing CopyControl™ Cloning Systems for Single-Copy Cloning and "On-Demand" Induction of Clones to High Copy Number
• The CopyControl™ Fosmid Library Production Kit Generates More Complete and Unbiased (Blunt-End) Libraries of Stable, 40-Kb Genomic Clones
• Save At Least 2 Days Constructing a CopyControl™ BAC Library Then Amplify the Clones to 10 - 20 Copies Per Cell, for Higher DNA Yields and Purity
• The CopyControl™ PCR Cloning Kits Enable Faster, More Reliable Cloning of Any PCR Product Up to 15 Kb - Even If It Has High A-T, G-C, or Repetitive Sequences, or Encodes a Toxic Peptide
• Retrofit Existing BAC and Fosmid Clones with CopyControl™ Capability Using a Kit for Simple In Vitro Insertion of an OriV-containing EZ::TN™ Transposon
• BAC-Tracker™ Supercoiled DNA Ladder
• TransforMax™ EPI300™ E. coli
• Electroporation Cuvettes
• Obtain PCR-Ready Genomic DNA from Buccal Cells, HeLa Cells, Hair Follicles, Tail Snips, Bacterial Cells, or Feather Using the QuickExtract™ DNA Extraction Solution
• Transposon Mutagenesis in the Parasitic Protozoa Trypanosoma brucei with the EZ::TN™ Transposome™
• In Vitro Insertion of a Transposon Containing an E. coli Origin of Replication Facilitates Rapid Recovery, Propagation, and Sequencing of Circular DNA Molecules from Heterologous Organisms
• DNA Ligation in 5 Minutes!

2001

Forum 8 . 3［PDF 1.59MB］

• The BuccalAmp™ DNA Extraction Kit: A New Tool for Easier, Faster Sample Collection and Extraction of PCR-Ready Genomic DNA from Large Numbers of Samples
• Get Consistent PCR of Templates 20 Kb to >40 Kb Using the MasterAmp™ Extra-Long PCR System
• Prepare Custom EZ::TN™ Transposons by PCR Using Primers with Transposase-Specific Mosaic End (ME) Sequences
• Cloning-Ready Bacterial Artificial Chromosome (BAC) Vectors
• Get >4X106 BAC Clones/μg DNA Using TransforMax™ EC100™ Electrocompetent E. coli
• Screen Your BAC Clones in 3 Hours and Plasmid Clones in 1 Hour Without Minipreps and Restriction Digests Using the Colony Fast-Screen™ Kit
• Stop Cloning or Subcloning Artifacts!
• Simplify Dephosphorylation of Your Cloning Vectors Using Heat-Labile HK™ Phosphatase
• Sequencing Through GC-rich and AT-rich Sequences with the SequiTherm™ EXCEL™ II DNA Sequencing Kit
• Produce Unbiased Libraries of Stable, Cosmid-Sized Clones Using the EpiFos™ Fosmid Library Production Kit
• Efficiently Clone a PCR Product into the Vector of Your Choice Using the End-It™ DNA End-Repair Kit
• Direct PCR from a Single Bacterial Colony Without DNA Extraction Using the FailSafe™ PCR System
• Get the Highest Yields of Yeast DNA Using the MasterPure™ Yeast DNA Purification Kit
• Create Random Gene Knockouts in Living Cells Using the EZ::TN™ Transposome™
• Rapidly Rescue Clone Transposed Genomic DNA
• DNA Ligation in 5 Minutes! At room temperature.

Forum 8 . 2［PDF 1.65MB］

• EZ::TN™ Transposomes™: A Novel System for Identifying Genes Involved in Bacterial Pathogenesis
• Direct PCR from Dried Blood without DNA Extraction Using the FailSafe™ PCR System
• High Fidelity PCR Amplification of DNA from 20 Kb to >40 Kb Using the MasterAmp™ Extra-Long PCR Kit
• Cloning and Identification of Expressed Plant Defense Genes Following RT-PCR Using the MasterAmp™ High-Fidelity RT-PCR Kit and Degenerate Primers
• New BuccalAmp™ DNA Extraction Kit Revolutionizes Buccal Sample Collection and Processing
• Completely Remove Oligonucleotides and Single-Stranded DNA From Your Reaction Mixes Using Exonuclease I
• Produce Single-Stranded PCR Product for SSCP or Sequencing Using Lambda Exonuclease
• Consistent Production of the Highest RNA Yields with AmpliScribe™ High Yield Transcription Kits
• Highest Yield of 5’-Capped RNA from an In Vitro Transcription Reaction with AmpliCap™ High Yield Message Maker Kits
• Gentle and Quantitative Recovery of Large Genomic DNA Fragments from Agarose Gels for Genomic Cloning
• The Highest Transformation Efficiency Electrocompetent E. coli Cells
• pWEB™ and pWEB::TNC™ Cosmid Cloning Kits and EpiFOS™ Fosmid Library Production Kit
• Use of the EZ::TN™ Transposon Insertion System for High-Throughput Full-Length cDNA Sequencing at the NIH Intramural Sequencing Center (NISC)
• The Most Versatile Transposition System
• DNA Ligation in 5 Minutes! At room temperature.

Forum 8 . 1［PDF 3.23MB］

• Identification of Permissive and Non-Permissive Peptide Insertion Sites in beta-galactosidase using the EZ::TN™ In-Frame Linker Insertion Kit
• Finish your DNA Sequencing Project: Complete Difficult Regions with SequiTherm EXCEL™ II DNA Sequencing Kits
• Rapid Extraction of PCR-Ready DNA from Buccal Cells Using the BuccalAmp™ DNA Extraction Kit
• High Fidelity PCR Amplification of DNA up to 40 Kb Using the MasterAmp™ Extra-Long PCR Kit
• Purification of DNA from Food Materials for Detection of Genetically Modified Organisms (GMOs) Using the MasterPure™ DNA Purification Kit
• RiboShredder™ RNase Blend Destroys Unwanted RNA Quickly and Efficiently
• pIndigoBAC-5 Cloning-Ready Vectors are Extensively Tested for High Cloning Efficiency and Low Background
• Three Ways to Increase Protein Yield for High Throughput Screening, Preparative Purification, or Bioprocessing
• Simultaneous SNP Identification in p53 and HPV Viral Typing with Multiplex Base Excision Sequence Scanning (BESS)
• Efficiently Clone a PCR Product into the Vector of Your Choice Using the End-It™ DNA End-Repair Kit
• DNA Ligation in 5 Minutes, Then Screen Your Clones in 1 Hour Using the Colony Fast-Screen™ Kit
• Produce Unbiased Libraries of Single Copy Cosmid-Sized Clones Using the EpiFos™ Fosmid Library Production Kit
• Whatever the Template, You Will Never Fail at PCR Again. We Promise.

2000

Forum 7 . 4［PDF 1.40MB］

• Direct Sequencing of BAC Clones Without Subcloning or Primer Walking Using EZ::TN™ Transposon Tools
• How to Make Sequencing Faster and Easier Using EZ::TN™ Transposon Tools
• EZ::TN™ Transposon Insertions Into Target DNA In Vitro are Highly Random
• Construction of an Environmental Genomic DNA Library from Soil Using the EpiFOS™ Fosmid Library Production Kit
• New BuccalAmp™ DNA Extraction Kit Revolutionizes Buccal Sample Collection and Processing!
• Cloning Ready BAC Vectors
• EZ::TN™ pMOD™-2<MCS> Transposon Construction Vector Fequently Asked Questions
• Direct PCR from Whole Blood
• Obtain the Highest Transformation Efficiency Possible Using TransforMax™ EC100™ Electrocompetent E. coli
• Rescue Cloning of Bacterial Genomic DNA Using the EZ::TN™ <R6Kgamma-ori /KAN-2> Tnp Transposome™
• EZ::TN™ <R6Kgamma-ori /KAN-2> Insertion Kit
• Electrocompetent E. coli for Rescue Cloning
• DNA Ligation in 5 Minutes at Room Temperature!

Forum 7 . 3［PDF 888KB］

• High-Throughput Screening of Single Nucleotide Polymorphisms (SNPs) Using the BESS-T&G™ Base Reader Kit and the ABI PRISM® 3700 DNA Sequencer
• Protein Modification Using the New EZ::TN™ In-Frame Linker Insertion Kit
• New Transposon Construction Vector for Constructing a Custom EZ::TN™ Transposon
• Recover Intact DNA Up to >2 Mb in Length with GELase™ Agarose Gel-Digesting Preparation
• Exonuclease I: Highest Activity, Available Now!
• Using the FailSafe™ PCR System to Construct Molecular Tools for the Functional Characterization of a Saccharomyces cerevisiae Gene
• The MasterAmp™ RT-PCR Kit for High Sensitivity Outperforms Other Kits for Sensitive Detection of RNA
• High Efficiency Packaging of Methylated DNA for Genomic Library Construction Using MaxPlax™ Lambda Packaging Extracts
• New High Efficiency TransforMax™ EC100 Electrocompetent E. coli
• DNA Ligation in as little as 5 minutes at room temperature with the Fast-Link™ DNA Ligation Kit

Forum 7 . 2［PDF 1.11MB］

• Using the EZ::TN™ Transposome™ for Transposon Mutagenesis in Mycobacterium smegmatis
• Finish Your DNA Sequencing Project: Complete Difficult Regions with SequiTherm™ EXCEL II DNA Sequencing Kits
• EZ::TN™ Transposome™ Frequently Asked Questions
• Consistent Production of the Highest Possible RNA Yields with Ampliscribe™ High Yield Transcription Kits
• FailSafe™ PCR: A New System For Reliable and Consistent Amplification of Both Routine and Challenging Templates
• EZ::TN ™ Transposon Tools: EZ::TN™ <T7 /KAN-2> Promoter Insertion Kit and EZ::TN™ In-Frame Linker Insertion Kit
• GELase™ Agarose Gel-Digesting Preparation and Plasmid-Safe™ ATP-Dependent DNase
• MaxPlax™ Lambda Packaging Extracts

Forum 7 . 1［PDF 1.41MB］

• Generating Unidirectional Deletions by In Vitro Transposition for Localizing and Sequencing a Cloned Gene using EZ::TN™ Transposon Tools
• Get the Highest Possible Yield of Full Length RNA with AmpliScribe™
• Highly Efficient Recovery of DNA from Dried Blood Using the MasterPure™ Complete DNA and RNA Purification Kit
• Recover Intact DNA 50 Kb to >2 Mb in Length with GELase™ Agarose Gel-Digesting Preparation
• Two Unique Kits for All Your RT-PCR Needs!
• Detection of Heterozygous Mutations in Hereditary Colorectal Cancer Using the BESS-T™ Base Reader Kit
• DNA Ligation in as Little as 5 Minutes with Fast-Link™ DNA Ligation Kits

1999

Forum 6 . 3［PDF 300KB］

• Use of EZ::TN™ Transposomes™ for Genetic Analysis and Direct Sequencing of Bacterial Genomic DNA
• Get the Highest Possible Yield of Full Length RNA!
• Ultra-Sensitive Purification of Microbial Nucleic Acids Using the MasterPure™ Complete DNA and RNA Purification Kit
• The New MasterAmp™ High Fidelity RT-PCR Kit for Accurate and Flexible RT-PCR
• Superior RT-PCR Fidelity with the MasterAmp™ High Fidelity RT-PCR Kit

Forum 6 . 2［PDF 1.54MB］

• FailSafe™ PCR: A New System For Reliable and Consistent Amplification of Both Routine and Challenging Templates
• New Transposon Tools for Genomics and Proteomics Research
• EZ::TN™ Transposon Tools
• Epidemiological Typing of Viruses Using the BESS-T™ Base Reader Kit: Identification of Genetic Variation Among St. Louis Encephalitis Virus Isolates Using an ABI Automated DNA Sequencer
• Amplification of Human Papillomavirus (HPV) DNA From Gynecological Specimens Isolated Using MasterAmp™ DNA Extraction Solution and PreservCyt® Solution
• FailSafe™ PCR System

Forum 6 . 1［PDF 1.41MB］

• The New MasterPure™ Plant Leaf DNA Purification Kit: Use in Grapevine DNA STR Analysis
• Using MasterAmp™ PCR PreMixes to Optimize Analysis of High GC Content Genes: PCR Amplification of the Melanocortin-1 Receptor Gene
• Faster Genotyping of Transgenic Mice with the MasterPure™ DNA Purification Kit and MasterAmp™ PCR Products
• New Products

1998

Forum 5 . 4［PDF 1.45MB］

• Demystifying DNA Yields from Yeast: Introducing the MasterPure™ Yeast DNA Purification Kit
• Efficient Fractionation of Periplasmic, Cytoplasmic, and Membrane Proteins From Escherichia coli Using the EPICENTRE PeriPreps™ Periplasting Kit
• Detection of the Differential Expression of Chorionic Gonadotropin by Multiplex RNA Amplification Using the MasterAmp™ RT-PCR Kit
• Detection of a Single-Base Substitution in the Human Calcium Sensing Receptor Gene Using the BESS T-Scan™ Mutation Detection and Localization Kit with an ABI DNA Sequencer
• Q&A: BESS Mutation Detection and Localization Kits

Forum 5 . 3［PDF 1.44MB］

• High-Efficiency Cosmid Library Construction Using the New pWEB™ Cosmid Cloning Kit
• Detection of Blood-Borne Microbes with the MasterPure™ Complete DNA and RNA Purification Kit
• Multiplex PCR Amplification of STRs from DNA Isolated with the MasterAmp™ Buccal Swab DNA Extraction Kit
• Isolation of DNA from Paraffin-Embedded Tissue Using the MasterPure™ Complete DNA and RNA Purification Kit

Forum 5 . 2［PDF 2.50MB］

• Single-Nucleotide DNA Fingerprinting of Hepatitis C Virus Using the BESS T-Scan™ and New BESS G-Tracker™ Mutation Detection and Localization Kits
• Analysis of BESS T-Scan™ Mutation Data Using an ABI Automated DNA Sequencer
• Single-Nucleotide Typing of Mycobacterium Species Using the BESS T-Scan™ Mutation Detection and Localization Kit
• The MasterPure™ Complete DNA and RNA Purification Kit: A Flexible System for Rapid Isolation of DNA and RNA from a Wide Variety of Sources

Forum 5 . 1［PDF 1.49MB］

• One-Step cDNA Synthesis and Amplification with the New MasterAmp™ RT-PCR Kit
• Convenient, Rapid Enrichment of Periplasmic and Spheroplasmic Protein Fractions Using the New PeriPreps™ Periplasting Kit
• Degrading Protein in DNA Samples to Ensure Proper Migration in Agarose Gels

1997

Forum 4 . 4［PDF 1.64MB］

• Detection of Human DNA Mutations Using Base Excision Sequence Scanning (BESS) and the LI-COR® IR2 Automated DNA Sequencer
• Use of the MasterAmp™ PCR Optmization Kit in Multiplex PCR to Detect Dystrophin Gene Deletions Characteristic of Duchenne and Becker Muscular Dystrophies
• Rapid Isolation of PCR-Ready DNA for Prenatal Diagnosis Using the MasterAmp™ Buccal Swab DNA Extraction Solution
• In-Gel PCR: DNA Amplification in the Presence of Agarose

Forum 4 . 3［PDF 2.39MB］

• Isolation of Intact, High Molecular Weight DNA Fragments for the E. coli Genome Project
• The ReadyPreps™ Protein Preparation Kit Facilitates Protein Isolation from Bacterial Cells
• The SequiTherm EXCEL™ II DNA Sequencing Kit: A New Kit for Both Cycle and Non-Cycle Sequencing

Forum 4 . 2［PDF 1.99MB］

• Simple, Rapid, Nucleotide-Level Characterization of Sequence Variations Using the BESS T-Scan™ Mutation Detection and Localization Kit
• Increased Sensitivity in DNA Sequencing with the New SequiTherm EXCEL™ II DNA Polymerase
• Sensitive and Efficient PCR Amplification of DNA with AmpliTherm™ DNA Polymerase
• A One-Step MasterAmp™ RT-PCR Procedure to Detect the BCR/ABL Transcript of the Philadelphia Chromosome and the Enterovirus Genome

Forum 4 . 1［PDF 1.31MB］

• New MasterAmp™ PCR Enhancement Technology Consistently Amplifies Even Very Difficult Templates
• Convenient and Highly Reproducible Amplification of DNA from Difficult Templates Using the MasterAmp™ PCR Optimization Kit
• Sensitive and Flexible Amplification of DNA Using the MasterAmp™ PCR Core Kit, Including Amplification of Difficult Templates
• Use of the MasterAmp™ Buccal Swab DNA Extraction Kit for Genomic Marker Amplification
• Rapid Purification of Genomic DNA from Buffy Coat Using the MasterPure™ Genomic DNA Purification Kit
• Optimal Conditions for Digesting Genomic DNA with T4 Endonuclease V
• Resolving Ambiguities in Automated Sequencing Data with the IsoTherm™ DNA Sequencing Kit

1996

Forum 3 . 4［PDF 1.35MB］

• The Direct Sequencing of CCG Repeats from a Cosmid Genomic DNA Template
• An Efficient and Inexpensive Method for Mutant Detection in Mice Transgenic for Bacteriophage Lambda
• Use of RNase I for the Efficient Elimination of RNA from DNA Preparations and Mismatch Detection
• Specificity and Heat Lability of Plasmid-Safe™ ATP-Dependent DNase

Forum 3 . 3［PDF 1.53MB］

• New SequiTherm™ EXCEL™ DNA Sequencing Kits Read Through Even Difficult Template Regions and Decrease Background in DNA Sequencing
• T4 Endonuclease V Detects UV Transilluminator Damage to DNA in Agarose Gels
• High Efficiency Ligation Using the Fast-Link™ DNA Ligation and Screening Kit
• The Advantages of EPICENTRE's MMLV-RT for Making Full-Length, Double-Stranded cDNA Templates
• Experimental Relevance of the Observed Two-Fold Higher Activity of EPICENTRE's Thermostable DNA Polymerases
• A Simple Method for RNA Purification from In Vitro Transcription Reactions

Forum 3 . 2［PDF 1.39MB］

• Non-Radioactive In Situ Hybridization Using Digoxigenin-labeled RNA Probes Generated with the AmpliScribe™ T7, SP6, and T3 High Yield Transcription Kits
• Rapid Cloning and Identification of Recombinant Plasmids Using the [Fast-Link™] DNA Ligation and Screening Kit
• A Single Buffer for Multiple Manipulations of DNA Samples
• Tech Tips: DNA Sequencing

Forum 3 . 1［PDF 1.22MB］

• Molecular Chaperones - An Overview
• Tech Tips: Nucleic Acid Ligation
• Protocol Update: Ampligase® Thermostable DNA Ligase for Gene Synthesis
• Selecting the Best Product for Optimum Results in In Vitro Transcription
• Irreversible Heat Inactivation of HK™-UNG Thermolabile Uracil N-Glycosylase

1995

Forum 2 . 5［PDF 1.28MB］

• High Efficiency Transgenic DNA Rescue Using the MutaPlax™ Transgenic Lambda DNA Packaging System
• Production of Biotin-Labeled RNA Probes Using the AmpliScribe™ T7 High Yield Transcription Kit
• DNA Sequencing in a Microtiter Plate Using the IsoTherm™ DNA Sequencing Kit for End-Labeling
• Sequencing a Double-Stranded Clone of a Mutant Human Complement Factor H Gene Using the IsoTherm™ DNA Sequencing Kit for Internal Labeling

Forum 2 . 4［PDF 1.29MB］

• Sequencing a 2.5 Kb CT-Rich Region of the Human Autosomal Dominant Polycystic Kidney (PKD1) Disease Gene Using the SequiTherm™ Long-Read™ Cycle Sequencing Kit
• Mutagenesis of Amplified DNA Sequencing Using Ampligase® Thermostable DNA Ligase
• Tech Tips: Rapid Isolation and Cloning of DNA Inserts
• Tech Tips: Isolation of Size-Specific RNA from Total Yeast RNA Using GELase™ Agarose Gel-Digesting Preparation
• IsoTherm™ DNA Sequencing Kits: New Kit Configuration!

Forum 2 . 3［PDF 1.20MB］

• Repeat Expansion Detection Using Ampligase® Thermostable DNA Ligase
• Obtaining Maximum Packaging Efficiencies in Transgenic DNA Rescue
• Preparation of Competent Cells Using Transformation and Storage Solution
• Tech Tips: Direct Sequencing of PCR Products from an Agarose Gel using the SequiTherm™ Cycle Sequencing Kit

Forum 2 . 2［PDF 1.19MB］

• Rapid Gene Synthesis Using Ampligase® Thermostable DNA Ligase
• Increased Efficiency of Rescue of a Lambda Shuttle Vector from Transgenic Mouse DNA using MaxPlax™ Packaging Extract
• Tech Tips: A Rapid and Efficient Method for Screening Bacterial Colonies for Recombinant Plasmids

Forum 2 . 1［PDF 1.45MB］

• MaxPlax™ Packaging Extract: The Highest Efficiency System for DNA Packaging
• DNase-Free Thermolabile DNA Phosphatase
• Direct Sequencing of P1 Bacteriophage Clones Using SequiTherm™ DNA Polymerase
• Isolation of High Molecular Weight DNA from Soybeans Using GELase™ Gel-Digesting Preparation
• Tech Tips: DNA-Specific Nucleases
• Sequencing "Difficult" DNA Templates

1994

Forum 1 . 4［PDF 1.30MB］

• Use of GELase™ Gel-Digesting Preparation for Improved EM of High Molecular Weight DNA Extracted from an Agarose Gel
• Intermolecular Ligation Using T4 RNA Ligase
• Improved Yields of DNA, RNA, and Proteins with Ready-Lyse™ Lysozyme Solution
• Dephosporylation of RNA Using HK™ Thermolabile Phosphatase
• Tech Tips: AmpliScribe™ T7, SP6, and T3 High Yield Transcription Kits

Forum 1 . 3［PDF 1.46MB］

• Use of GELase™ Gel-Digesting Preparation in Constructing Chromosome-Specific YAC Libraries
• IsoTherm™ DNA Polymerase and IsoTherm DNA Sequencing Kits
• T4 RNA Ligase
• Thermal Cycle Sequencing Directly from Bacterial Colonies Using SequiTherm™ DNA Polymerase
• Sequencing GC-Rich Templates Using SequiTherm™ DNA Polymerase
• Tech Tips: SequiTherm™ Cycle Sequencing Kit for Manual Sequencing of DNA

Forum 1 . 2［PDF 1.46MB］

• Unmodified T7 DNA Polymerase for the In Situ Detection of DNA Fragmentation Associated with Apoptosis
• Cycle Sequencing Using SequiTherm™ with [alpha-33P]-Nucleotides
• Tobacco Acid Pyrophosphatase to Remove Caps from mRNA
• Use of Plasmid-Safe™ DNase to Prevent Cloning Artifacts Due to Bacterial Chromosomal DNA
• A Simple Method of RNA Purification from In Vitro Transcription Reactions
• Tech Tips: GELase™ Agarose Gel-Digesting Preparation
• GELase™: How to Do the Fast Protocol Even Faster

Forum 1 . 1［PDF 1.55MB］

• SequiTherm™ Long-Read™ Kit for High Forum DNA Sequencing: Use of a Modified M13 DNA Preparation with SequiTherm DNA Polymerase for Fluorescence Sequencing Reactions
• Automated DNA Sequencing Using the SequiTherm™ Long-Read™ Kit
• AmpliScribe™ for Synthesis of Active Ribozymes in Milligram Quantities
• Tech Tips: Plasmid-Safe™ ATP-Dependent DNase
• Cycle Sequencing of Unpurified PCR-Generated DNA Using a New Thermostable DNA Polymerase

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