MasterAmp™ Extra-Long PCR Kit
MasterAmp™ Extra-Long PCR Kitは20-40 kbのDNAを増幅させるシステムです。MasterAmp™ Extra-Long DNA Polymerase Mixは、3´→5´proofreading機能を持つMasterAmp™ Taq DNA Polymeraseを採用しています。キットには9種類のMasterAmp Extra-Long PCR 2X PreMixes (dNTPs, buffer, 様々な濃度のMgCl2とMasterAmp™ PCR Enhancer with Betaine*)がついています。
Two Steps to Reliable and Consistent Extra-Long PCR:
STEP 1
PCRを行いたいテンプレートとプライマー及びMasterAmp™ Extra-Long DNA Polymerase Mixと9種類のPreMixesを用いて、最適なPreMixを調べるためにPCRを行います。
Figure 1. Amplification of a 20-kb region of lambda DNA using MasterAmp Extra-Long PCR 2X PreMixes (1-9).
MasterAmp Extra-Long PCR PreMix 4 produced optimal results. M = DNA size marker.
STEP 2
Step1にて得られた結果にてMasterAmp™ Extra-Long PCR PreMixを選択することで一貫したPCRの結果が得られます。(Figure 2).
Figure 2. Eight individual amplifications of a 20-kb region of lambda DNA using MasterAmp™ Extra-Long PCR PreMix 4.
Subsequent PCR results are consistent and reliable using the PreMix identified in Step 1.
Applications
- ~40 kbのHigh-fidelity PCRが行えます。
Benefits
- Fast optimization-Extra-Long PCR 2X PreMixes with MasterAmp™ PCR Enhancer, as well as a robust and high fidelity DNA polymerase mix, makes achieving optimized extra-long PCR easy.
*Covered by issued and pending patents.
| 商品名 | Cat.No. | Size |
| MasterAmp™ Extra-Long PCR Kit | MHF9220 | 50 Reactions |
Kit Contents
- MasterAmp™ Extra-Long PCR PreMixes 1-9
- MasterAmp™ Extra-Long DNA Polymerase Mix
- Control Lambda DNA/Primers
| 商品名 | Cat.No. | Size |
| MasterAmp™ Extra-Long PCR 2X Premix 1 | MHF925A | 5 ml |
| MasterAmp™ Extra-Long PCR 2X Premix 2 | MHF925B | 5 ml |
| MasterAmp™ Extra-Long PCR 2X Premix 3 | MHF925C | 5 ml |
| MasterAmp™ Extra-Long PCR 2X Premix 4 | MHF925D | 5 ml |
| MasterAmp™ Extra-Long PCR 2X Premix 5 | MHF925E | 5 ml |
| MasterAmp™ Extra-Long PCR 2X Premix 6 | MHF925F | 5 ml |
| MasterAmp™ Extra-Long PCR 2X Premix 7 | MHF925G | 5 ml |
| MasterAmp™ Extra-Long PCR 2X Premix 8 | MHF925H | 5 ml |
| MasterAmp™ Extra-Long PCR 2X Premix 9 | MHF925I | 5 ml |
| 商品名 | Cat.No. | Concentration | Size |
| MasterAmp™ Extra-Long DNA Polymerase Mix (Enzyme Mix Only) | QU92125 | 2.5 U/μl | 125 Units |
| QU92500 | 2.5 U/μl | 500 Units | |
| QU9201K | 2.5 U/μl | 1,000 Units |
製品のご購入・ご相談はTEL:03-3545-5720、またはお問い合わせまで。

